Báo cáo khoa học: A Kazal prolyl endopeptidase inhibitor isolated from the skin of Phyllomedusa sauvagii
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Searching for bioactive peptides, we analyzed acidic extractsofPhyllomedusa sauvagiiskin and found two new proteins,PSKP-1 and PSKP-2, of 6.7 and 6.6 kDa, respectively,which, by sequence homology, belong to the Kazal familyof serine protease inhibitors. PSKP-1 and PSKP-2 exhibitthe unprecedented feature of having proline at P1and P2positions. A gene encoding PSKP-1 was synthesized andexpressed in Escherichia coli. Recombinant PSKP-1 waspurified from inclusion bodies, oxidatively refolded to thenative state, and characterized by chemical, hydrodynamicandoptical studies. ...
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Báo cáo khoa học: A Kazal prolyl endopeptidase inhibitor isolated from the skin of Phyllomedusa sauvagii
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: A Kazal prolyl endopeptidase inhibitor isolated from the skin of Phyllomedusa sauvagii
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