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Báo cáo khoa học: Comparative analysis of the site-specific N-glycosylation of human lactoferrin produced in maize and tobacco plants

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We have compared the site-by-siteN-glycosylation status ofhuman lactoferrin (Lf) produced in maize, a monocotyle-don, and in tobacco, usedas amodel dicotyledon.Maizeandtobacco plants were stably transformed and recombinant Lfwas purified from both seeds and leaves. N-glycopeptideswere generated by trypsin digestion of recombinant Lf andpurified by reverse-phase HPLC. The N-glycosylation pat-tern of each site was determined by mass spectrometry.Our results indicated that the N-glycosylation patterns ofrecombinant Lf produced in maize and tobacco sharecommon structural features....
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Báo cáo khoa học: Comparative analysis of the site-specific N-glycosylation of human lactoferrin produced in maize and tobacco plants

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