Báo cáo khoa học: Do N-terminal nucleophile hydrolases indeed have a single amino acid catalytic center? Supporting amino acid residues at the active site of penicillin G acylase
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A new set of experimental kinetic data on the hydrolysis of a series ofphenylacetylp-substituted anilides catalyzed by penicillin G acylase fromEscherichia coli(PGA) is presented in this article. The Hammett plot oflog(kcat,R⁄kcat,H) versus rp)has three linear segments, which distinguishesthe enzyme from the other N-terminal nucleophile hydrolases for whichdata are available.
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Báo cáo khoa học: Do N-terminal nucleophile hydrolases indeed have a single amino acid catalytic center? Supporting amino acid residues at the active site of penicillin G acylase
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Báo cáo khoa học: Do N-terminal nucleophile hydrolases indeed have a single amino acid catalytic center? Supporting amino acid residues at the active site of penicillin G acylase
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