Báo cáo khoa học: Limited proteolysis of Escherichia coli cytidine 5¢-triphosphate synthase. Identification of residues required for CTP formation and GTP-dependent activation of glutamine hydrolysis
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Cytidine 5¢-triphosphate synthase catalyses the ATP-dependent formation of CTP from UTP using eitherammonia orL-glutamine as the source of nitrogen. Whenglutamine is the substrate, GTP is required as an allostericeffector to promote catalysis. Limited trypsin-catalysedproteolysis, Edman degradation, and site-directed muta-genesis were used to identify peptide bonds C-terminal tothree basic residues (Lys187, Arg429, and Lys432) ofEscherichia coliCTP synthase thatwere highly susceptible toproteolysis. ...
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Báo cáo khoa học: Limited proteolysis of Escherichia coli cytidine 5¢-triphosphate synthase. Identification of residues required for CTP formation and GTP-dependent activation of glutamine hydrolysis
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: Limited proteolysis of Escherichia coli cytidine 5¢-triphosphate synthase. Identification of residues required for CTP formation and GTP-dependent activation of glutamine hydrolysis
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