Báo cáo khoa học: Proteolytic processing of porcine deoxyribonuclease II occurs in lysosomes but is not required for enzyme activation
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DNase II purified from porcine spleen (pDNase II) comprisesa1, a2 andbsubunits. The three subunits are encoded by one cDNA, in the sequencea1, b, and a2, and the peptides linking these subunits are presumablycleaved out post-translationally. To understand the relevance of post-trans-lational cleavage to pDNase II, recombinant pDNase II (rpDNase II) wasproduced in human 293T cells by transfection with an expression plasmidcontaining pDNase II cDNA (pcDNaseII).
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: Proteolytic processing of porcine deoxyribonuclease II occurs in lysosomes but is not required for enzyme activation
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: Proteolytic processing of porcine deoxyribonuclease II occurs in lysosomes but is not required for enzyme activation
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Scientific reports scientific research biochemistry Structural biochemistry oxidation bacteriaTài liệu liên quan:
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