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Báo cáo khoa học: Rate-determining step of butyrylcholinesterase-catalyzed hydrolysis of benzoylcholine and benzoylthiocholine Volumetric study of wild-type and D70G mutant behaviou

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The rate-limiting step for hydrolysis of thepositivelychargedoxoester benzoylcholine (BzCh) by human butyrylcholinesterase (BuChE) is deacylation (k3), whereas it is acyla-tion (k2) for hydrolysis of the homologous thioester ben-zoylthiocholine (BzSCh). Steady-state hydrolysis of BzChand BzSCh by wild-type BuChEand its peripheral anionicsite mutant D70G was investigated at different hydrostaticpressures, which allowed determination of volume changesassociated with substrate binding, and the activation vol-umes for the chemical steps....
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Báo cáo khoa học: Rate-determining step of butyrylcholinesterase-catalyzed hydrolysis of benzoylcholine and benzoylthiocholine Volumetric study of wild-type and D70G mutant behaviou

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