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Báo cáo khoa học: Selection of stably folded proteins by phage-display with proteolysis

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To facilitate the process of proteindesign and learn the basicrules that control the structure and stability of proteins,combinatorial methods have been developed to select orscreen proteins with desired properties from libraries ofmutants. One such method uses phage-display and proteo-lysis to select stably folded proteins. This method does notrely on specific properties of proteins for selection. There-fore, in principle it can be applied to any protein. Since itsfirst demonstration in 1998, the method has been used tocreate hyperthermophilic proteins, to evolve novel foldeddomains froma librarygeneratedby combinatorial shufflingof polypeptide segments and to convert a partially unfoldedstructure to a fully folded protein....
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Báo cáo khoa học: Selection of stably folded proteins by phage-display with proteolysis

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