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Báo cáo khoa học: Transport of taurocholate by mutants of negatively charged amino acids, cysteines, and threonines of the rat liver sodium-dependent taurocholate cotransporting polypeptide Ntcp

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The relevance of functional amino acids for taurocholatetransport by the sodium-dependent taurocholate cotrans-porting polypeptide Ntcp was determined by site-directedmutagenesis. cRNAfrom28 single-pointsmutants of the ratliver Ntcp clone was expressed in Xenopus laevisoocytes.Mutations were generated in five conserved negativelychargedaminoacids (aspartates andglutamates)whichwerepresent in nine members of the SBAT-family, in two non-conserved negatively charged amino acids, in all eight Ntcp-cysteines, and in two threonines from a protein kinase Cconsensus region of theNtcpC-terminus. ...
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Báo cáo khoa học: Transport of taurocholate by mutants of negatively charged amino acids, cysteines, and threonines of the rat liver sodium-dependent taurocholate cotransporting polypeptide Ntcp

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