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Báo cáo khoa học: Usefulness of microchip electrophoresis for reliable analyses of nonstandard DNA samples and subsequent on-chip enzymatic digestion

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The Hitachi SV1100 utilizes capillary electrophoresis on amicrochip that is capable of rapidly sizing DNAfrag-ments. Reproducibility of electrophoresis in differentchannels was shown by comparing the migration times ofthe internal controls, DNAfragments of 100 and 800 bp.The range of DNAsizing for this microchip is between100 and 800 bp, and accuracy in sizing of a 322 bp DNAfragment of a pUC118 PvuII digest was observed, inde-pendent of DNAconcentration. Although relatively goodquantification of this fragment was observed with a DNAconcentration of 1.83 ngÆlL)1, error increased in a dose-dependent manner. ...
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Báo cáo khoa học: Usefulness of microchip electrophoresis for reliable analyses of nonstandard DNA samples and subsequent on-chip enzymatic digestion

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