Báo cáo sinh học: Permissive human cytomegalovirus infection of a first trimester extravillous cytotrophoblast cell line

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Permissive human cytomegalovirus infection of a first trimester extravillous cytotrophoblast cell line
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Báo cáo sinh học: " Permissive human cytomegalovirus infection of a first trimester extravillous cytotrophoblast cell line"Virology Journal BioMed Central Open AccessShort reportPermissive human cytomegalovirus infection of a first trimesterextravillous cytotrophoblast cell lineHeather L LaMarca1,2, Bruno Sainz Jr2 and Cindy A Morris*1,2Address: 1Interdisciplinary Program in Molecular and Cellular Biology, Tulane University Health Sciences Center, New Orleans, LA, USA and2Department of Microbiology and Immunology, Tulane University Health Sciences Center, New Orleans, LA, USAEmail: Heather L LaMarca - hlamarc@tulane.edu; Bruno Sainz - bsainz@tulane.edu; Cindy A Morris* - cmorris2@tulane.edu* Corresponding authorPublished: 17 November 2004 Received: 02 September 2004 Accepted: 17 November 2004Virology Journal 2004, 1:8 doi:10.1186/1743-422X-1-8This article is available from: http://www.virologyj.com/content/1/1/8© 2004 LaMarca et al; licensee BioMed Central Ltd.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0),which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Human cytomegalovirus (HCMV) is the leading cause of congenital viral infection in the United States and Europe. Despite the significant morbidity associated with prenatal HCMV infection, little is known about how the virus infects the fetus during pregnancy. To date, primary human cytotrophoblasts (CTBs) have been utilized to study placental HCMV infection and replication; however, the minimal mitotic potential of these cells restricts experimentation to a few days, which may be problematic for mechanistic studies of the slow-replicating virus. The aim of this study was to determine whether the human first trimester CTB cell line SGHPL-4 was permissive for HCMV infection and therefore could overcome such limitations. HCMV immediate early (IE) protein expression was detected as early as 3 hours post-infection in SGHPL-4 cells and progressively increased as a function of time. HCMV growth assays revealed the presence of infectious virus in both cell lysates and culture supernatants, indicating that viral replication and the release of progeny virus occurred. Compared to human fibroblasts, viral replication was delayed in CTBs, consistent with previous studies reporting delayed viral kinetics in HCMV-infected primary CTBs. These results indicate that SGHPL-4 cells are fully permissive for the complete HCMV replicative cycle. Our findings suggest that these cells may serve as useful tools for future mechanistic studies of HCMV pathogenesis during early pregnancy. lous cytotrophoblasts (CTBs), which are specialized pla-Findings cental epithelial cells that invade and remodel the uterineHuman cytomegalovirus (HCMV) is a ubiquitous beta- wall during placentation, have been previously shown toherpesvirus that is the leading cause of congenital viral be fully permissive for HCMV infection in vitro [7,9].infection in the United States and Europe. Intrauterine Additionally, using an in vitro coculture system, Maidjitransmission of the virus occurs in approximately 40% of and colleagues demonstrated that infected uterine micro-pregnant women with primary HCMV infection, and the vascular endothelial cells transmit HCMV to differentiat-incidence of congenital HCMV infection is an estimated ing invading CTBs, suggesting that placental HCMV1% of newborns [1-3]. Although the pathogenesis of infection can occur in a retrograde fashion that initiates inHCMV transmission to the fetus during pregnancy is the maternal endothelium [8]. Despite these reports, theunclear, the placenta has been implicated as an important minimal mitotic potential of primary CTBs restricts exper-determining factor [4-8]. Primary first trimester extravil- imentation to a few days, which may be problematic for Page 1 of 4 (page number not for citation purposes)Virology Journal 2004, 1:8 http://www.virologyj.com/content/1/1/8 A 100 % IE Positive Cells 80 60 40 ...