Báo cáo Y học: A comparative biochemical and structural analysis of the intracellular chorismate mutase (Rv0948c) from Mycobacterium tuberculosis H37Rv and the secreted chorismate mutase (y2828) from Yersinia pestis
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The Rv0948c gene fromMycobacterium tuberculosisH37Rv encodes a 90amino acid protein as the natural gene product with chorismate mutase(CM) activity. The protein, 90-MtCM, exhibits Michaelis–Menten kineticswith akcat of 5.5 ± 0.2 s)1and a Kmof 1500 ± 100lmat 37C andpH 7.5. The 2.0 A˚X-ray structure shows that 90-MtCM is an alla-helicalhomodimer (Protein Data Bank ID: 2QBV) with the topology ofEscheri-chia coliCM (EcCM), and that both protomers contribute to each catalyticsite. Superimposition onto the structure of EcCM and the sequence align-ment shows that the C-terminus helix 3 is shortened....
Nội dung trích xuất từ tài liệu:
Báo cáo Y học: A comparative biochemical and structural analysis of the intracellular chorismate mutase (Rv0948c) from Mycobacterium tuberculosis H37Rv and the secreted chorismate mutase (y2828) from Yersinia pestis
Nội dung trích xuất từ tài liệu:
Báo cáo Y học: A comparative biochemical and structural analysis of the intracellular chorismate mutase (Rv0948c) from Mycobacterium tuberculosis H37Rv and the secreted chorismate mutase (y2828) from Yersinia pestis
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