Identification and characterization of drought-responsive CC-type glutaredoxins from cassava cultivars reveals their involvement in ABA signalling

CC-type glutaredoxins (GRXs) are plant-specific glutaredoxin, play regulatory roles in response of biotic and abiotic stress. However, it is not clear whether the CC-type GRXs are involve in drought response in cassava (Manihot esculenta), an important tropical tuber root crop.
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Identification and characterization of drought-responsive CC-type glutaredoxins from cassava cultivars reveals their involvement in ABA signallingRuan et al. BMC Plant Biology (2018) 18:329https://doi.org/10.1186/s12870-018-1528-6 RESEARCH ARTICLE Open AccessIdentification and characterization ofdrought-responsive CC-type glutaredoxinsfrom cassava cultivars reveals theirinvolvement in ABA signallingMeng-Bin Ruan1,5*† , Yi-Ling Yang2†, Kai-Mian Li3, Xin Guo4, Bin Wang4, Xiao-Ling Yu1,5 and Ming Peng1,5 Abstract Background: CC-type glutaredoxins (GRXs) are plant-specific glutaredoxin, play regulatory roles in response of biotic and abiotic stress. However, it is not clear whether the CC-type GRXs are involve in drought response in cassava (Manihot esculenta), an important tropical tuber root crop. Results: Herein, genome-wide analysis identified 18 CC-type GRXs in the cassava genome, of which six (namely MeGRXC3, C4, C7, C14, C15, and C18) were induced by drought stress in leaves of two cassava cultivars Argentina 7 (Arg7) and South China 124 (SC124). Exogenous abscisic acid (ABA) application induced the expression of all the six CC-type GRXs in leaves of both Arg7 and SC124 plants. Overexpression of MeGRXC15 in Arabidopsis (Col-0) increases tolerance of ABA on the sealed agar plates, but results in drought hypersensitivity in soil-grown plants. The results of microarray assays show that MeGRXC15 overexpression affected the expression of a set of transcription factors which involve in stress response, ABA, and JA/ET signalling pathway. The results of protein interaction analysis show that MeGRXC15 can interact with TGA5 from Arabidopsis and MeTGA074 from cassava. Conclusions: CC-type glutaredoxins play regulatory roles in cassava response to drought possibly through ABA signalling pathway. Keywords: Cassava (Manihot esculenta), Drought response, ABA, CC-type glutaredoxin, Transgenic Arabidopsis, TGA factorsBackground delays leaf senescence under drought stress [3–5]. It isAs a tropical crop, cassava (Manihot esculenta) evolved dif- therefore necessary to analyze genes involved in these path-ferent responses to drought stress, such as quick stomata ways for a deeper functional characterization.closure, reduction of photosynthetic proteins levels and Glutaredoxin (GRX) is one of the most important pro-photosynthetic capacity, induction of senescence in older tein modification system in plants [6]. The glutathione/leaves, and size reduction of leave epidermal cells [1, 2]. GRX (GSH/GRX) system is essential for redox homeosta-The cassava cultivar Argentina 7 (Arg7) display faster sen- sis and ROS signalling in plant cells [7]. GRX target pro-escence in older leaves than the cassava cultivar South teins are involved in all aspects of plant growth, includingChina 124 (SC124) [2]. Senescence in cassava is partly con- basal metabolism, iron/sulfur cluster formation, develop-trolled by reactive oxygen species (ROS) and ethylene (ET) ment, adaptation to the environment, and stress responsessignaling [3]. Increasing ROS scavenging ability in cassava [7]. GRX are in particular studied for their involvement in oxidative stress responses [7–9]. GRXs are classified in* Correspondence: ruanmengbin@itbb.org.cn five subgroups, among which CC-type GRXs are a† Meng-Bin Ruan and Yi-Ling Yang contributed equally to this work. plant-specific subgroup, also known as the ROXY family1 Institute of Tropical Bioscience and Biotechnology, Chinese Academy of in Arabidopsis [10, 11]. CC-type GRXs likely evolved fromTropical Agricultural Sciences, Haikou 571101, China5 Key Laboratory of Biology and Genetic Resources of Torpical Crops, Ministry the CPYC subgroup and expanded during land plant evo-of Agriculture, Haikou 571101, China lution [11]. There are only two CC-type GRXs in the basalFull list of author information is available at the end of the article © The Author(s). 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.Ruan et al. BMC Plant Biology (2018) 18:329 Page 2 of 17land plant Physcomitrella, but between 15 and 24 mem- Resultsbers in land plants such as rice, Arabidopsis, Vitis and Phylogenetic and protein sequence analysis of cassavaPopulus [11]. However, the number of CC-type ...