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Cystatin F is a recently discovered type II cystatin expressed almost exclu-sively in immune cells. It is present intracellularly in lysosome-like vesicles,which suggests a potential role in regulating papain-like cathepsins involvedin antigen presentation. Therefore, interactions of cystatin F with severalof its potential targets, cathepsins F, K, V, S, H, X and C, were studiedin vitro. Cystatin F tightly inhibited cathepsins F, K and V with Ki valuesranging from 0.17 nmto 0.35 nm, whereas cathepsins S and H were inhib-ited with 100-fold lower affinities (Ki 30 nm). The exopeptidases, cathep-sins C and X were not inhibited by cystatin F....
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Báo cáo khoa học: Inhibitory properties of cystatin F and its localization in U937 promonocyte cells
