Cell Metabolism Cell Homeostasis and Stress Response Part 15

Sự ra đời của sinh học bắt đầu từ thế kỉ 19, khi các nhà khoa học tìm thấy được các đặc điểm chung cơ bản giữa các loài. Ngày nay, sinh học trở thành một môn học chuẩn và bắt buộc tại các trường học và Đại học trên khắp thế giới, và rất nhiều bài báo được công bố hằng năm ở trên khắp các tạp chí chuyên ngành về y và sinh. [2]
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Cell Metabolism Cell Homeostasis and Stress Response Part 15 201Imaging Cellular Metabolismaddress such questions, it will be important to analyze cell cycle dependent events in largenumbers of cells. A very promising new technique for measuring cell cycle dependentgrowth was demonstrated recently, using spatial light interference microscopy (SLIM)coupled with a fluorescence marker for S-phase to analyze cell cycle phase within a cellpopulation (Mir et al., 2011). The applications for this technique to a range of cell types, aswell as to microscopy systems that utilize multi-channel fluorescence imaging, open endlesspossibilities for developing variations on this method to image cellular metabolism in thecontext of cell growth even within a complex cellular population.5. ConclusionThe rapid progress recently made toward developing metabolic tracer molecules showsgreat promise for new applications in clinical diagnostics. Further characterization of novelimaging probes is needed to understand how they can be used to image and identifymalignant tissues. Rapidly screening novel tracer molecules for efficacy in identifyingtumors in cell culture systems, animal models and clinical trials is a crucial ongoingchallenge aimed toward building a battery of tools for imaging cancer metabolism inpatients. Feeding into clinical studies is a vast amount of knowledge gained from basicresearch characterizing metabolic pathways in single cells. This information has potentialfor wide use for diagnostic imaging, but awaits further research and development intotranslational medicine that will utilize novel biomarkers and imaging technologies. Finally,continued development of super-resolution imaging platforms for both basic research andclinical use are certain to have a major impact on our understanding of molecularcomplexes, especially with regard to colocalization of specific protein-protein, protein-RNAor protein-DNA complexes within the overall context of cellular architecture.6. ReferencesAmiel, A., Litmanovitch T., Lishner M., Mor A., Gaber E., Tangi I., Fejgin M. & Avivi, L. 1998. Temporal differences in replication timing of homologous loci in malignant cells derived from CML and lymphoma patients. Genes Chromosomes Cancer 22: 225–231.Andersen, J.S., Lam Y.W. Leung A.K.L., Ong S._E., Lyon C., Lamond A.I., & Mann M. 2004. Nuclolar proteome dynamics. Nature. 433:77-83.Barwick, T., Bencherif B., Mountz J.M. & Avril N. 2009. Molecular PET and PET/CT imaging of tumour cell proliferation using F-18 fluoro-L-thymidine: a comprehensive evaluation. Nucl. Med. Commun. 30: 908-17.Ben-Ari, Y., Brody Y., Kinor N., Mor A., Tsukamoto T., Spector D.L., Singer R.H. & Shav-Tal Y. 2010. The life of an mRNA in space and time. J Cell Sci. 123: 1761-1774.Ben-Haim, S. & Ell P. 2009. 18F-FDG PET and PET/CT in the evaluation of cancer treatment response. J. Nucl. Med. 50: 88-99.Bolzer A., Kreth G., Solovei I., Koehler D., Saracoglu K., Fauth C., Muller S., Eils R., Cremer C., Speicher M.R. & Cremer, T. (2005) Three-Dimensional Maps of All Chromosomes in Human Male Fibroblast Nuclei and Prometaphase Rosettes. PLoS Biol 3(5): 157.202 Cell Metabolism – Cell Homeostasis and Stress ResponseBoisvert, F.M., Hedzel M.J. & Bazett-Jones, D.P.. 2000. Promyelocytic leukemia (PML) nuclear bodies are protein structures that do not accumulate RNA. J. Cell Biol. 148: 283-292.Bouchier-Hayes, L., Oberst A., McStay G.P., Connell S., Tait S.W.G., Dillon C.P., Flanagan J.M., Beere H.M. & Green D.R. 2009. Characterization of cytoplasmic caspase-2 activation by induced proximity. Mol. Cell. 25: 830-840.Chopra, A., Shan L., Eckelman W.C., Leung K., Latterner M., Bryant S.H. & Menkens A. 2011. Molecular Imaging and Contrast Agent Database (MICAD): Evolution and Progress. [Internet]. Mol. Imaging Biol. Oct 12.Comar, D., Cartron J.C., Maziere M. & Marazano, C. 1976. Labelling and metabolism of methionine-methyl-11C. Eur. J. Nucl. Med. 1: 11-14.Condeelis, J. &Weissleder R. 2010. In vivo imaging in cancer. Cold Spring Harb. Perspect. Biol. 2:a003848.Cremer, T., Kurz A., Zirbel R., Dietzel S., Rinke B., Schrock E., Speicher M.R., Mathieu U., Jauch A., Emmerich P., Scherthan H., Reid T., Cremer C. & Lichter, P. 1993. Role of chromosome territories in the functional compartmentalization of the cell nucleus. Cold Spring Harbor Symp. Quant. Biol. 58: 777–792.Deng, H., Tang X., Wang H., Tang G., Wen F., Shi X., Yi C., Wu K. & Meng Q. 2011. S-11C- methyl-L-cysteine: a new amino acid PET tracer for cancer imaging. J. Nucl. Med. 52: 287-93.Dimitrova, D.S. 2011. ...