Investigation of antioxidant activity of Zoanthus Vietnamensis's crude extract and dichloromethane extract

Zoanthid is a potential natural resource with many biological activities, such as antioxidant, antiviral, antiinflammatory, and cytotoxic activity. Therefore, this study aimed to evaluate the antioxidant activity of 70% ethanol crude extract and dichloromethane fraction from Zoanthus vietnamensis. The total polyphenol content of ethanol and dichloromethane extracts were 19.36 ± 1.37 and 24.95 ± 0.63 mg GAE/g extract, respectively.
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Investigation of antioxidant activity of Zoanthus Vietnamensis’s crude extract and dichloromethane extract Vietnam Journal of Marine Science and Technology 2023, 23(4) 419–427 Vietnam Academy of Science and Technology Vietnam Journal of Marine Science and Technology journal homepage: vjs.ac.vn/index.php/jmstInvestigation of antioxidant activity of Zoanthus vietnamensis’s crudeextract and dichloromethane extractPhan Bao Vy*, Le Ho Khanh Hy, Nguyen Phuong Anh, Doan Thi Thiet, Pham Xuan Ky,Dao Viet HaInstitute of Oceanography, VAST, VietnamReceived: 22 April 2023; Accepted: 23 June 2023ABSTRACTZoanthid is a potential natural resource with many biological activities, such as antioxidant, antiviral, anti-inflammatory, and cytotoxic activity. Therefore, this study aimed to evaluate the antioxidant activity of 70%ethanol crude extract and dichloromethane fraction from Zoanthus vietnamensis. The total polyphenolcontent of ethanol and dichloromethane extracts were 19.36 ± 1.37 and 24.95 ± 0.63 mg GAE/g extract,respectively. The DPPH free radical scavenging capacity increased slightly from low to high concentration.The IC50 values reached 9.974 µg/mL and 6.424 µg/mL for ethanol crude and dichloromethane extract. Thepotassium ferricyanide-reducing power assay result was meager, and the optical density value ranges from0.015 to 0.105.Keywords: Antioxidant activity, ethanol crude extract, dichloromethane extract, Zoanthus vietnamensis.* Corresponding author at: Institute of Oceanography, 01 Cau Da St., Nha Trang city 650000, Khanh Hoa, Vietnam.E-mail addresses: baovyphan@gmail.comhttps://doi.org/10.15625/1859-3097/18293ISSN 1859-3097; e-ISSN 2815-5904/© 2023 Vietnam Academy of Science and Technology (VAST) 419 Phan Bao Vy et al./Vietnam Journal of Marine Science and Technology 2023, 23(4) 419–427INTRODUCTION lichens. The chemical structure of MAAs has changed the position of amino acids to reach Marine resources in coral reefs are some MAAs with different physical andabundant, especially in the phylum Cnidaria. chemical properties leading to adapting to harshThis group has more than 10,000 species, such environmental conditions and becoming newas Anthozoa (sea anemones, corals, and antioxidant compounds [8]. According tozoanthids), Scyphozoa (jellyfishes), Cubozoa Dunlap and Yamamoto (1995), the antioxidant(box jellyfishes), Hydrozoa and Staurozoa [1]. activity of aqueous extracts from four marineThey are considered potential raw materials in species, including grouper Plectropomusmedicine, chemistry, and cosmetics. leopardus, red seaweed Porphyra tenera, squid The biological activities of this group have Lissoclinum patella, and coral Palythoabeen studied, for example, anti-inflammatory, tuberculosa, was determined by inhibition ofanti-microbial, antioxidant, cytotoxic, and anti- phosphatidylcholine peroxidation. The resultscancer. According to Roy et al., (2012), the showed that the most potent antioxidant activitysecondary metabolites from an Okinawan soft was the extract from Palythoa tuberculosa thatcoral Cespitularia sp. were identified as five the mycosporine-glycine was in their structurenovel diterpenoids and one alcyonolide. The [9]. Moreover, Xuan Cuong et al. evaluatedresults showed that the alcyonolide was antioxidant activity on some fractions, such as n-cytotoxic to HCT 116 cell line (IC50 value was butanol, ethyl acetate, n-hexane, ethanol, andabout 5.85 µM), and diterpenoids were less chloroform from Aaptos suberitoides. Theactive than the previous compound (IC50 value chloroform fractions had the strongestranged from 28.2 to 91.4 µM) [2]. In addition, antioxidant activity, three times higher than thealmost all active compounds from Vietnamese crude ethanol extract (75.36%) [5].sponges, for example, Aaptos suberitoides, The zoanthid was a typical group in coralPetrosia nigricans, Dysidea cinerea, Dysidea reefs, in which the dominant genus like as,fragilis, Smenospongia cerebriformis, and Zoanthus, Protopalythoa, and Palythoa becauseHaliclona oculate were alkaloids, terpenoids, of the strong competitiveness, highand steroids with biological activities, such as adaptability, combination of asexual and sexualanti-inflammatory, antibacterial, antitumor, and reproduction, and rapid growth. This group wascytotoxic activity [3–5]. evaluated as a rich source with n ...