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The gene encoding an integrase of Mason–Pfizer monkey virus (M-PMV)is located at the 3¢-end of the pol open reading frame. The M-PMV integ-rase has not been previously isolated and characterized. We have nowcloned, expressed, isolated, and characterized M-PMV integrase and com-pared its activities and primary structure with those of HIV-1 and otherretroviral integrases. M-PMV integrase prefers untranslated 3¢-region-derived long-terminal repeat sequences in both the 3¢-processing and thestrand transfer activity assays. While the 3¢-processing reaction catalyzedby M-PMV integrase was significantly increased in the presence ofMn2+and Co2+and was readily detectable in the presence of Mg2+andNi2+cations, the strand transfer activity was strictly dependent...
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Báo cáo khoa học: Integrase of Mason–Pfizer monkey virus
