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Báo cáo Y học: Distinct parts of minichromosome maintenance protein 2 associate with histone H3/H4 and RNA polymerase II holoenzyme

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10.10.2023

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Minichromosomemaintenance (MCM) proteins are part ofthe replication licensing factor (RLF-M), which limits theinitiationofDNAreplication to once per cell cycle.We havepreviously reported that higher order complexes of mam-malian pol II and general pol II transcription factors,referred to as pol II holoenzyme, also contain MCM pro-teins. In the present study we have analyzed in detail theinteraction between MCM2 and pol II holoenzyme. N- andC- terminal deletions were introduced into epitope-taggedMCM2 and the truncated proteins were transientlyexpressed in 293 cells. ...
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Báo cáo Y học: Distinct parts of minichromosome maintenance protein 2 associate with histone H3/H4 and RNA polymerase II holoenzyme

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